![]() ![]() Perform the susceptibility test by a disc-diffusion method using standard methods described in the guidelines. Characteristics E.coli Catalase test Positive Oxidase test Negative Nitrate reduction test Positive Methyl-Red (MR) test Positive Voges-Proskauer (VP) test Negative Citrate utilization test Negative Acetate utilization test Positive Indole test Positive Pyrrolidonyl-β-naphthylamide (PYR) test Negative H2S production test No Urease test Negative Oxidative-fermentative (OF) test Fermentative MUG test Positive TSI reactions Acid/Acid, Gas, SIM No H 2S, Indole +ve, motile Motility Motile Phenyl Pyruvic acid (PPA) test Negative Lysine decarboxylation test + Arginine decarboxylation test -/+ (strain variability) Ornithine decarboxylation test +/- (strain variability) ONPG + Sugar fermentation test Glucose Yes Sucrose No Lactose Yes Mannitol Yes Antimicrobial susceptibility testing
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